Description
This Mouse Chemokine Ligand 9 (CCL9) Rapid ELISA Kit is intended is a fast ELISA kit, this assay takes less than 1.5 hours to detect Mouse Mip 1 Gamma/Ccl9 with <1pg/ml sensitivity. format: 96-well plate with removable strips. Compatible samples: cell culture supernates, cell lysates, serum and plasma (heparin, EDTA). This is a TMB colorimetric sandwich ELISA kit with short assay time and fast experiment set up. Mip 1 Gamma/Ccl9 tissue specificity: Expressed mainly in the liver, lung, and the thymus, although some expression has been is a fast ELISA kit, this assay takes less than 1.5 hours to detected in a wide variety of tissues except brain. Strip well format. Reagents for up to 96 tests.
This mouse CCL9 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for CCL9 has been precoated onto 96-well plates. Standards (E Coli, Q22-Q122) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CCL9 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse CCL9 amount of sample captured in plate.
The capture antibody is monoclonal antibody from rat, the detection antibody is polyclonal antibody from goat. Expression system for standard: Chemokine (C-C motif) ligand 9 (CCL9), also called MIP-1 gamma and MRP-2, is a small cytokine belonging to the CC chemokine family. CCL9 which found around Peyer's patches is secreted by follicle-associated epithelium (FAE), and it can attract dendritic cells that possess the cell surface molecule CD11b and the chemokine receptor CCR1. CCL9 is constitutively expressed in macrophages and myeloid cells. CCL9 can activate osteoclasts through its receptor CCR1 (the most abundant chemokine receptor found on osteoclasts) suggesting an important role for CCL9 in bone resorption